replifactory: a multiplexed, automated, and scalable platform for continuous evolution experiments

Contents:

• Introduction
  • What is Replifactory?
• Device Calibration
  • Enter Calibration Mode
  • Pump Calibration
  • Stirrer Calibration
  • OD Sensor Calibration
• Experiment Setup Guide
  • Initial Setup
  • Stock Bottles Preparation
  • Priming the Tubing
  • Setting Up Experiment Parameters
  • Starting the Experiment
  • Monitoring the Experiment
• Culture Control Parameters
  • Growth Rate and Doubling Time
• Troubleshooting